Part:BBa_K3045002

pGBSS-Xa-GFP

This plasmid contains: - 35S promoter - DNA coding region consisting GBSS (granule-bound starch synthase), factor Xa cleavage site and GFP. - Nopaline synthase terminator. This system allows us to produce plants expressing a target protein (GFP) fused to GBSS. This is a simple and cost-effective plant-based method for production and purification of recombinant proteins.

The part number BBa_K304502 was constructed using two existing Parts from the Registry of Standard Biological Parts [BBa_K1467101 (35S promoter) and BBa_K1484215 (nopaline synthase terminator)] and a new Part (BBa_K304501) that contains a DNA coding region consisting GBSS (granule-bound starch synthase)-factor Xa cleavage site-GFP.

Evaluation of their functionality was conducted by analyzing the presence of GFP into the starch granules of Arabidopsis leaves. As we can see in the figure these existing Parts that we have used are perfectly functional.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 392
  Illegal BglII site found at 1138
  Illegal BglII site found at 1601
  Illegal BglII site found at 1640
  Illegal BamHI site found at 1976
  Illegal BamHI site found at 3037
  Illegal XhoI site found at 1342
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 4015
  Illegal BsaI.rc site found at 4302